Discovering Protein-Coding Genes from the Environment: Time for the Eukaryotes?
Trends Biotechnol. 2017 Sep;35(9):824-835
Authors: Marmeisse R, Kellner H, Fraissinet-Tachet L, Luis P
Eukaryotic microorganisms from diverse environments encompass a large number of taxa, many of them still unknown to science. One strategy to mine these organisms for genes of biotechnological relevance is to use a pool of eukaryotic mRNA directly extracted from environmental samples. Recent reports demonstrate that the resulting metatranscriptomic cDNA libraries can be screened by expression in yeast for a wide range of genes and functions from many of the different eukaryotic taxa. In combination with novel emerging high-throughput technologies, we anticipate that this approach should contribute to exploring the functional diversity of the eukaryotic microbiota.
PMID: 28279485 [PubMed – indexed for MEDLINE]