Detecting Antigen-Specific T Cell Responses: From Bulk Populations to Single Cells.

Figure 1

Analysis antigen-specific T cells at the single cell level pipeline. (A) Detection of antigen-specific CD4+ and CD8+ T cells using multiple assays and MHC-multimers; (B) Functional analysis of single Ag-specific cells via micro-well dense arrays and nanolitre arrays or single cell trapping using microfluidic valves for further molecular extraction/amplification; (C) Transcriptomic analysis using digital PCR and next-generation sequencing technologies (i.e., RNA-seq) (coloured lines and dots represent fluorescence signals).

Int J Mol Sci. 2015 August;16(8):18878-18893.

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