Next-generation sequencing fragment library construction.

All current next-generation sequencing (NGS) platforms and applications require the sequencing library to have specific characteristics: in particular, size, size distribution, and 5′ and 3′ flanking sequences. This unit presents a robust protocol for converting a wide variety of input DNA samples into appropriate NGS libraries and discusses important considerations in experimental design, failure modes, and typical results.

Copyright © 2014 John Wiley & Sons, Inc.

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