RNA structure, binding, and coordination in Arabidopsis.
Wiley Interdiscip Rev RNA. 2017 Jun 28;:
Authors: Foley SW, Kramer MC, Gregory BD
From the moment of transcription, up through degradation, each RNA transcript is bound by an ever-changing cohort of RNA binding proteins. The binding of these proteins is regulated by both the primary RNA sequence, as well as the intramolecular RNA folding, or secondary structure, of the transcript. Thus, RNA secondary structure regulates many post-transcriptional processes. With the advent of next generation sequencing, several techniques have been developed to generate global landscapes of both RNA-protein interactions and RNA secondary structure. In this review, we describe the current state of the field detailing techniques to globally interrogate RNA secondary structure and/or RNA-protein interaction sites, as well as our current understanding of these features in the transcriptome of the model plant Arabidopsis thaliana. For further resources related to this article, please visit the WIREs website.
PMID: 28660659 [PubMed – as supplied by publisher]