The Role of Next-Generation Sequencing in the Cytologic Diagnosis of Pancreatic Lesions.

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The Role of Next-Generation Sequencing in the Cytologic Diagnosis of Pancreatic Lesions.

Arch Pathol Lab Med. 2018 Apr;142(4):458-464

Authors: de Biase D, Visani M, Acquaviva G, Fornelli A, Masetti M, Fabbri C, Pession A, Tallini G

Abstract
CONTEXT: – Integration of the analysis of genetic markers with endoscopic ultrasound-guided fine-needle aspiration and cytologic evaluation has increased the accuracy of the preoperative diagnosis of pancreatic lesions. The application of high-throughput gene panel analysis using next-generation sequencing platforms is now offering a great opportunity for further improvements.
OBJECTIVE: – To review the application of next-generation sequencing to the preoperative diagnosis of pancreatic lesions.
DATA SOURCES: – For data acquisition, a PubMed search using the terms next-generation sequencing, pancreas, pancreatic lesions, pancreatic tumors, and EUS-FNA was performed covering the years 2000-2017.
CONCLUSIONS: – KRAS remains the gene most widely studied for preoperative single-gene tests. Next-generation sequencing reliably allows analysis of multiple gene markers starting from limited amounts of DNA. The study of multigene panels has become a very attractive option for the management and preoperative risk stratification of patients with pancreatic cancer.

PMID: 29565213 [PubMed – in process]

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