Polymerase chain reaction (PCR)-based methods: Promising molecular tools in dentistry.
Int J Biol Macromol. 2018 May 17;:
Authors: Shahi S, Vahed SZ, Fathi N, Sharifi S
Polymerase chain reaction (PCR) has become a popular diagnosis and research technique in dentistry. Several studies show that its high sensitivity and specificity allow it as a precise, efficient, and rapid method for detection, identification, and quantification of microorganism. Several genetic polymorphisms can be determined along with detection of immune and inflammatory markers, therefore providing the more accurate perception into the mechanisms underlying the dental and periodontal disease. This review paper discusses the application of PCR as a diagnostic technique in periodontology, endodontic infections, implant-related, and peri-implantitis infection, immune and inflammatory markers identification and genetic polymorphism as well as application of next generation sequencing and gene microarray technology in dentistry mentioned. The manuscript was prepared mainly using PubMed and MEDLINE.
PMID: 29778881 [PubMed – as supplied by publisher]